HBV infection upregulates the PD-L expression on HepG 2.2.15 cell
- VernacularTitle:乙型肝炎病毒感染上调2.2.15细胞系程序性死亡分子配体表达的研究
- Author:
Yu HE
;
Xianjie YU
;
Ji CHEN
;
Guiqiang WANG
- Publication Type:Journal Article
- Keywords:
HBV;
PD-1;
PD-L1/L2;
hepatocye
- From:
Chinese Journal of Practical Internal Medicine
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of programmed cell death-1(PD-1)ligands on HepG2 cell and 2.2.15 cell.Methods Culture HepG2 cell and 2.2.15 cell in vitro and stain the cells with fluorescence-labeled monoclonal antibodies specifically against PD-L1 and PD-L2,respectively.Isolate total RNA of HepG2 cell and 2.2.15 cell and detect the mRNA expressions of PD-L1 and PD-L2 by RT-PCR.Results The expression of PD-L1 on 2.2.15 cell was upregulated on the levels of both proteins and mRNA.The expression of PD-L2 in 2.2.15 cell was not detected.And neither the PD-L1 nor PD-L2 was detected in HepG2 cell.Conclusion HBV infection can upregulate the expression of PD-L1 on hepatocyte,which might contribute to the immune evasion of HBV.
