The fitting and optimization of standard curve for determining concentration of serum hepatitis B virus large surface protein with program solution of Excel
- VernacularTitle:Excel软件规划求解功能拟合与优选血清乙型肝炎病毒大蛋白质量浓度标准曲线
- Author:
Aiping LE
;
Taiyuan LI
;
Guoxin HU
;
Zhongqin LUO
;
Qingshui HUANG
- Publication Type:Journal Article
- Keywords:
hepatitis virus,B;
large surface protein;
enzym-linked immunosorbent assay;
standard curve;
regression model;
program solution;
coefficient of determination
- From:
Chinese Journal of Practical Internal Medicine
2006;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss a convenient and pragmatic method of fitting and optimizing standard curve for determining concentration of serum hepatitis B virus large surface protein(HBV-LP).MethodsEnzyme-linked immunosorbent assay(ELISA)was used to measure the absorbance of standard preparation of HBV-LP.Concentration and absorbance of standard preparation of HBV-LP was carried out curve fitting with 4-parameter formula model and linear model and log-linear model and quadratic polynomial model and cubic polynomial model and S model by program solution of Excel,respectively.The most standard curve for determining concentration of serum HBV-LP was determined with coefficient of determination of regression model.ResultsThe scatterplot of standard preparation of HBV-LP submited nonlinear tendency.There were all significance to regression equation of 4-parameter formula model and linear model and log-linear model and quadratic polynomial model and cubic polynomial model and S model(P
