Interstitial cells of Cajal regulates excitability of detrusor myocytes: structure evidence
- VernacularTitle:膀胱Cajal间质细胞对膀胱逼尿肌细胞兴奋调控的结构和功能特征的初步研究
- Author:
Lili DING
;
Qiang FANG
;
Jianli FENG
;
Bo SONG
- Publication Type:Journal Article
- Keywords:
interstitial cells of Cajal;
fluorescence redistribution after photobleaching;
detrusor myocyte
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of bladder interstitial cells of Cajal(ICCs) in regulating the excitability of detrusor myocytes.Methods 1)Ultrastructural connection between ICCs and detrusor myocytes was detected by transmission electron microscope(TEM).2) Fluorescence redistribution after photobleaching(FRAP) was carried out to detect the intercellular communication between detrusor myocytes and its neighboring ICCs.Results 1)Gap junction between the 2 kinds of cells was confirmed by TEM.2)After target detrusor myocyte was photobleached,fluorescence intensity in detrusor myocytes was recovered gradually.In the meantime,fluorescence intensity in the ICCs neighboring to the target detrusor myocyte was decreased.This indicates a signal transmission from the neighboring ICCs to the target detrusor myocyte.Conclusion Structural connection is seen between ICCs and detrusor myocytes.Excitability signals might be transferred from ICCs to detrusor myocyte.
