Transfection of maspin/PCR2.1 vector inhibits uPA and uPAR expressions in gastric cancer cells in vitro
- VernacularTitle:转染maspin cDNA在胃癌细胞中的表达及其对uPA和uPAR表达的影响
- Author:
Wenwen ZHOU
;
Yongfen YI
;
Aihua ZHENG
- Publication Type:Journal Article
- Keywords:
maspin;
PCR2.1 expression vector;
urokinase-type plasminogen activitor(uPA);
uPAR;
gastric cancer
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of maspin gene on metastasis and invasion of gastric cancer and its mechanism.Methods Maspin gene was ligated to the expression vector PCR2.1 with T4 DNA ligase after the PCR2.1 was digested by HindⅢ/XbaⅠ.The recombinant vector maspin/PCR2.1 was transfected into human gastric cancer cell lines MKN28 and SGC7901,then the mRNA and protein expression changes of maspin gene,uPA and uPAR were detected respectively by PT-PCR and Western blotting.Results After identified by digestion and sequencing,the reconstructed plasmid was confirmed to contain the correct and full nucleotide sequence of maspin gene.The expressions of maspin gene were up-regulated in MKN28 and SGC7901 cell lines,while the expressions of uPA and uPAR were down-regulated.Conclusion The expression vector maspin/PCR2.1 is constructed successfully and can be expressed in eukaryotic cells.Expressions of uPA and uPAR can be inhibited by maspin gene.
