Effect of cisplatin on toxicity and apoptosis of human luteinized granulose cells
- VernacularTitle:顺铂对人卵巢黄素化颗粒细胞的毒性及凋亡的影响
- Author:
Chunyan ZHANG
;
Yuanli HE
- Publication Type:Journal Article
- Keywords:
cisplatin;
human luteinized granulose cells;
apoptosis;
bcl-2;
bax
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of cisplatin(CDDP) on toxicity and apoptosis of human luteinized granulose cells.Method Human luteinized granulose cells were prepared from oocyte retrieval of fertilization procedure in vitro and treated with various concentrations of cisplatin(0,0.5,1.0,2.5,5.0 mg?L-1).The inhibitory rate of cells was examined by MTT assay.The change of nucleolus stained with Hochest 33258 was observed under fluorescent microscope,and bcl-2 and bax protein expressions were detected by flow cytometry(FCM).Results The results showed that CDDP inhibited the growth of ovarian granulose cells at the concentration of no less than 1 mg?L-1,in which Hochest 33258 staining demonstrated cell apoptosis.The expression of bax protein increased as compared with that of the control(P
