Expression of periostin and the effect of hydrocortisone on it in human fibroblasts of scar
- VernacularTitle:periostin在人瘢痕成纤维细胞中的表达及氢化可的松对其表达的影响
- Author:
Zhenhua SONG
;
Zelian QIN
- Publication Type:Journal Article
- Keywords:
Gene,periostin;
Fibroblasts;
Cicatrix,hypertrophic;
Keloid;
Hydrocortisone
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To probe into the role of periostin in the formation of scars and investigate its reaction to hydrocortisone.Methods: Primary fibroblasts were cultured in vitro,then RT-PCR and immunocytochemical technique were used to examine the expressions of mRNA and preotein of periostin respectively in 24 samples of keloid fibroblasts(KFb),hypertrophic scar fibroblasts(HFb) and normal skin fibroblasts(SFb).Results: The mRNA levels of periostin in KFb(1.645?0.549) and HFb(1.084?0.396) were both higher than that in SFb(0.274?0.215,P
