Expression of amphiregulin in human endometrium during the menstrual cycle
- VernacularTitle:双调蛋白在人体子宫内膜组织中的表达特征
- Author:
Yaoting GUI
;
Jianrong ZHANG
;
Wanqing LIANG
;
Zhiming CAI
- Publication Type:Journal Article
- Keywords:
Amphiregulin;
Endometrium;
Reverse transcriptase polymerase chain reaction;
In situ hybridization;
Immunohistochemistry
- From:
Journal of Peking University(Health Sciences)
2004;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of amphiregulin in human endometrium during the menstrual cycle.Methods: Endometrial tissues were collected from the patients undergoing hysterectomy or endometrial biopsy.Real-time RT-PCR,in situ hybridization and immunohistochemistry were used to detect the expression characteristics of amphiregulin in human endometrium in proliferative and secretory phases.Results: Real-time RT-PCR showed the expression of amphiregulin mRNA in secretory phase was 32 times that in proliferative phase.The results from in situ hybridization and immunohistochemistry showed that amphiregulin was located in the cytoplasm and mainly expressed in the gland of endometrium.The expressions of amphiregulin mRNA in proliferative and secretory phases were 0.54?0.22 and 2.96?0.47(P
