DISTRIBUTION OF MOLECULAR WEIGHT OF PEPTIDE PREPARED BY COMPLEX ENZYME AND THE TUMOR INHIBITION TEST
- VernacularTitle:酶法制备大豆多肽的分子量分布和抑瘤实验观察
- Author:
Fang LV
;
Sha MI
;
Shixian WANG
;
Hui JIANG
;
Jie WANG
;
Guoqiang GAO
- Publication Type:Journal Article
- Keywords:
soybean peptides;
molecular weight distribution;
tumor inhibitory effect
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the molecular weight distribution of soybean peptides prepared by complex enzyme hydrolysation and tumor inhibitory effect in vivo or in vitro. Method The soybean protein was enzymolyzed to soybean peptides by Flavourzyme, Alealase and neutral protease, at(50-55)℃, followed by separation, absorption,affination, ultra centrifuge and spray drying. The soybean peptides were passed Sephadex G25 column at 0.5ml/min with 0.1mol/L Tris-HCl buffer. The fragment of fluid collected was determined by UV at 280nm and HPLC. Then the molecular weight distribution was calculated. The inhibitory effect on ansplant glioma (G422) at dose 1.25, 2.5 and 5g/kg bw (ig daily for 11 d) was tested in mice and in Hep-2 in vitro. Results The peptides contents in three batches were 54.6%, 51.9%, 51.8% respeclively. The molecular weight
