UV-Visible Spectrophotometric Assay for Saikosaponins in Bupleurum Chinense DC
- VernacularTitle:紫外可见分光光度法测定柴胡中柴胡总皂苷的含量
- Author:
Jinbao PU
;
Yijuan HU
;
Weiqing LIANG
;
Junxian ZHENG
;
Kemin WEI
- Publication Type:Journal Article
- Keywords:
UV-Visible spectrophotometry;
Bupleurum chinense DC.;
Saikosaponins;
Content determination
- From:
Journal of Medical Research
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a simple and accurate method for the determination of saikosaponins in Bupleurum chinense DC. by UV-Visible Spectrophotometry. Methods The content of saikosaponins was determined by UV-Visible Spectrophotometry. The absorbency was measured at 536nm. Results The calibration curves was linear within 194~1940mg/L(r=0.9996). The average recovery was 97.35%,and the RSD was 1.02%(n=9). Conclusion The method was proved to be simple, precise and reproduciable. It can be used for the quantitative determination of B upleurum chinense DC.
