The Characteristics of the Species Distribution and Detection of Vancomycin Resistance Genotypes in Enterococci
- VernacularTitle:肠球菌感染菌种分布及耐万古霉素基因型研究
- Author:
Bing YAN
;
Jian SU
- Publication Type:Journal Article
- Keywords:
Vancomycin;
Resistance;
Enterococcus spp;
Phenotypes;
Genotypes
- From:
Journal of Medical Research
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the characteritics of the species distribution, vancomycin resistance phenotypes, and genotypes in Enterococci in Shenzhen Second People,s Hospital. Methods Screening vancomycin-resistant Enterococci (VRE) with agar screen, and detecting the phenotypes and genotypes of vancomycin resistance or intermediate enterococci with E test and multiple PCR-restriction fragment length polymorphism. Results Among 310 Enterococcus spp strains isolated f rom clinical samples, 235 strains (75.8 %) were Enterococcus faecalis, 71 strains (22.9 %)were E. f aecium, 3 strains (1.0%) were Enterococcus gallinarum, and 1 strains (0.3%) was Enterococcus casseliflavus. 7 strains were vancomycin-resistant or intermediate to Enterococci, vanA for 2 strains were detected, vanC1 for 3 strains were detected, vanC2/3 for 1 strains was detected, 1 strains was unknown. Their phenotypes confirmed to the genotypes. Conclusion 2 strains vanA were found. It is important for clinical laboratory to detect VRE with right methods to instruct rational application of antibiotics clinically and prevent VRE prevalence.
