Expression of Human Vascular Endothelial Growth Factor 165 in Ischemically Damaged Bind Limb in a Rabbit Model with Transplantation of Mesenchymal Stem Cells
- VernacularTitle:hVEGF_(165)在自体骨髓间充质干细胞移植治疗兔缺血后肢中的表达
- Author:
Jinkan DU
;
Bin WANG
;
Laigen SHEN
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
VEGF165;
Expression
- From:
Journal of Medical Research
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of Vascular endothelial growth factor 165 (VEGF165) in transplantation of MSCs to repair an ischemically damaged bind limb in a rabbit model.Methods MSCs were cultivated in vitro and transfected with non-replicative adenoviral vector with VEGF165 gene. Four weeks after treatment, Real-time PCR assessed the expression of VEGF165 in the damaged limb.Results It was confirmed that VEGF165 was successfully transfected into MSCs.Real-time PCR assessments revealed the expression of Vascular endothelial growth factor 165 (VEGF165) in 4 weeks after treatment. Conclusion VEGF165 can be transfected into MSCs and express in rabbit model of ischemic limb.
