Observation of HSP60 and Ras Protein in Gastric Mucosa of Atrophic Gastritis Induced by High-salt Hot Water in Rats Using Immune Fluorescence and Laser Scanning Confocal Microscope
- VernacularTitle:免疫荧光及激光共聚焦技术检测热盐水致大鼠萎缩性胃炎胃黏膜组织细胞HSP60及ras蛋白表达
- Author:
Lingxia ZHANG
;
Li ZHANG
;
Mei TAO
;
Ying SONG
;
Guangzhoui CAO
- Publication Type:Journal Article
- Keywords:
High-salt-hot water;
Gastric mucosa;
HSP60;
Ras;
Immunofluorescence;
Laser scanning confocaI microscopy
- From:
Journal of Medical Research
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between long-term high-salt water and the morbidity mechanism of chronic atrophic gastritis (CAG), The expression of HSP60 and ras protein were detected in the gastric mucosa of CAG rates induced by high-salt water.Methods The atrophic gastritis rat model was made by high-salt-hot water perfusion and the ultrastructure of gastric mucosa was observed by Laser Scanning ConfocaI Microscopy. Results There was no expression in gastric mucosa in normal group. The expression of HSP60 and ras was observed in the cell plasm of rats at 12 weeks. The higher expression was observed in the rats at 24, 32 and 65 weeks. Using laser scanning confocaI microscopy and immunofluorescence technique had observed coexistence of HSP60 and ras.Conclusion Long time high-salt-hot water can induce CAG and increasing the expression of HSP60 and ras. HSP60 and ras play an important role in the formation of atrophic gastritis.
