Inhibitory effect of RNA interference on WT1 gene expression in HL-60 cell line
- VernacularTitle:RNA干扰对HL-60白血病细胞株WT1基因表达的抑制作用
- Author:
Huiqing YU
;
Yao LIU
- Publication Type:Journal Article
- Keywords:
leukemia;
siRNA;
WT1
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of RNA interference(RNAi)on WT1 gene expression in HL-60 cell line.Methods Several pairs of small interfering RNA(siRNA)for WT1 gene was designed and transfected into HL-60 cells.WT1 gene expression was assessed by RT-PCR,and WT1 protein expression was tested by Western blot.Flow cytometry was used to measure the cell apoptosis.Results The expression rates of WT1 mRNA and the expression rates of WT1 protein were(23.62%?1.28%),(23.20%?1.04%),respectively in HL-60 WT1 siRNA-transfected cells,significantly lower than those of control cells(P
