Identification and analysis of effective compositions of Schistosoma japonicum 31-32 kDa proteins
- VernacularTitle:日本血吸虫生殖相关抗原31～32 kDa分子的质谱分析与鉴定
- Author:
Lin LI
;
Shiping WANG
;
Shuaifeng ZHOU
;
Shaomin HU
;
Zhuo HE
;
Dongmei GAO
;
Mingzhao FENG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Vaccine;
Proteomics;
Two-dimensional electrophoresis;
Mass spectrum
- From:
Chinese Journal of Schistosomiasis Control
1989;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify and analyze the effective compositions of Schistosoma japonicum 31-32 kDa proteins by using the techniques of proteomics.Methods The total proteins were prepared from 32-day adult worms of Schistosoma japonicum.After two-dimensional(2-D)gel electrophoresis,the distinct protein spots from 2-D gels were isolated and analyzed by MALDI-TOF-MS.Results A total of 13 protein spots,within the range of 31-32 kDa,were detected in the 2-D gels.Three of them had high homology with Actine-2 of S.mansoni,glycerol-3-phosphate dehydrogenase of S.japonicum and cathepsin B endopeptidase of S.mansoni.Conclusions The 31-32 kDa antigens contain 3 important antigens:actine-2,glycerol-3-phosphate dehydrogenase and cathepsin B endopeptidase,which have been demonstrated to have certain protective effect against S.japonicum.Our findings can facilitate the development of multi-epitope vaccine against S.japonicum.
