Effect of Hongqu on induction differentiation of bone marrow stromal cells to osteoblasts in rat
- VernacularTitle:红曲对大鼠骨髓基质细胞向成骨细胞诱导分化的影响
- Author:
Jianhua LU
;
Xudai QIAN
;
Jiang HUA
;
Weijia WANG
;
Xiaofei ZHU
- Publication Type:Journal Article
- Keywords:
Hongqu;
Serum pharmacology;
Rat;
Bone marrow stromal cell;
Osteoblast;
Experimental study
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of Chinese medicinal herb Hongqu on induction differentiation of bone marrow stromal cells to osteoblasts.Methods:60 SD rats were divided randomly into 6 groups: blank control group,Low,medium and high dose of Hongqu groups,Provastain group,Premarin group.Each group was given distilled water,Hongqu extraction,Provastain solution,and Premarin solution through intragastric administration.10 days later,killed the rats and took the rats’ blood to prepared medicated serum.Meanwhile,the bone marrow stromal cells which comes from femur and tibia of the 4-5 week’s rats were cultured in medicated serum culture-medium,and MTT,para-nitro-pheneye phosphate,and the tinctorial method of alizarin Bordeaux were used to observe the effect of Hongqu on such aspects as the proliferation,ALP expression,and mineralization tuberculation of marrow stromal cells induction differentiation to osteoblasts.Results: Mmedicated serum of Hongqucould obviously increace the quantity of marrow stromal cells induction differentiation to osteoblasts,the level of the ALP expression and mineralization tuberculation(P
