The study on complete Freud’s adjuvant of prevention of STZ-induced diabetes
- VernacularTitle:完全弗氏佐剂预防链尿菌素引起糖尿病的机制研究
- Author:
Hua BAI
;
Xueyi MA
;
Aitao GUO
- Publication Type:Journal Article
- Keywords:
Type 1 diabetets;
Fas;
FasL;
CFA
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find out the immunologic mechanism of complete Freud's adjuvant (CFA) when used for preventing from streptozotocin (STZ)-insulitis and STZ-induced diabetes mellitus. Methods 20 healthy Kunming mice were equally and randomly divided into A and B groups. The animals in group A was respectively injected once into the pad of hind foot and intraperitoneally injected with 50?L CFA, and the animals in group B was injected with 50?L sodium chloride. After 2 weeks, all mice in both groups were i.p. injected with STZ (40 mg?kg-1/d) for 5 days. All mice were killed 6 weeks after administration of CFA or sodium chloride. Diabetes was diagnosed if blood glucose level was more than 16.7mmol/L after 2 consecutive days. The incidence of diabetes was compared between the two groups before mice were killed. The expression of Fas/FasL on ? cells as well as on infiltrating T cells of islets was investigated by immunochemistry method. Results The incidences of diabetes mellitus and insulitis in group A and group B were 0 % vs 80% (P
