Expression of Axin in Peutz-Jeghers syndrome
- VernacularTitle:黑斑息肉综合征中Axin表达研究
- Author:
Oudong XIA
;
Yamei MA
;
Baoping WU
- Publication Type:Journal Article
- Keywords:
Peutz-Jeghers syndrome;
Axin;
immunohistochemistry;
reverse transcriptase polymerase chain reaction(RT-PCR)
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study Axin expression in colorectal adenocarcinoma, colorectal adenoma, Peutz-Jeghers syndrome (PJS) polyp, and normal intestinal mucosa at mRNA and protein levels, and to explore the role of Axin in the pathogenesis of PJS. Methods The experiment samples, which were from the exsected normal mucosa, PJS polyps, adenomatous polyps and colorectal adenocarcinoma between Jan, 2001 and Mar, 2008 in Nanfang Hospital, each includes 32 sapamples. Immunohistochemistry was adopted to detect the Axin protein expression in each sample distinctively. Results transcriptase -polymerse chain reaction (RT-PCR) was used to detected and compare the Axin mRNA expression in 16 samples of each group. Results Of the tissues in each group, the positive Axin protein expression was located in the endochylema. The nomal mucosa group showed the most intensive expressive intensity, the olorectalc adenocarcinoma group was the least intensive, with significant difference in each group(P
