Effects of TNF-? on receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) expression of human dental follicle cells in vitro
- VernacularTitle:TNF-?对体外培养人牙囊细胞表达RANKL、OPG的影响
- Author:
Yingchun BI
;
Zhu LIN
;
Zuolin JIN
- Publication Type:Journal Article
- Keywords:
dental sac;
tumor necrosis factor;
receptor activator of nuclear factor-kappa B ligand;
osteoprotegerin
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of different concentration of tumor necrosis factor-? (TNF-?) on expression of the receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) mRNA on human dental follicle cells in vitro, and investigate the role of TNF-? in osteoclast formation during tooth eruption. Methods The 5th passage of primary cultured human dental follicle cells were treated with 0 (control group), 5, 10, 25, 50 and 100ng/ml TNF-?, respectively, for 6 hours. Total RNA was then isolated from human dental follicle cells and subjected to RANKL and OPG mRNA assay by reverse transcription-polymerase chain reaction (RT-PCR) technique. The relative expression levels of RANKL and OPG mRNA were normalized to ?-actin gene expression. Results The mRNA expression of OPG in human dental follicle cells with 5ng/ml TNF-? treatment was down regulated significantly compared with that in control group (P
