The Purification of Astrocytes From Cerebral Cortex of SD Rats
- VernacularTitle:SD大鼠脑皮质星形胶质细胞的纯化
- Author:
Yanying QIAN
;
Huimin YANG
;
Rongyuan ZHENG
- Publication Type:Journal Article
- Keywords:
Astrocyte;
Purify;
Primary culture;
SD rat;
Glial fibrillary acidic protein
- From:
Journal of Medical Research
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To simply the method of how to obtain cultures rich in astrocytes from SD rat cerebral cortex which could be utilized in vitro experiments.Methods The neonatal rat cerebral cortex was made into suspension by mechanical dissociation,and then reduced other cells by differential velocity adherent technique,shaking in orbital shaker and passage of cultured cells.After purification,the cultured cells were identified by double immunofluorescence staining and SABC.Results We successfully obtain cultures rich in astrocytes and the proportion of astrocytes were more than 95%.Conclusion The method described above was reliable in obtaining the high purity astrocytes from neonatal rat cerebral cortex and double immunofluorescence staining was more vivid and direct.
