Effect of Oxalate on the Growth of Distal Renal Tubular Epithelial Cells.
- Author:
Kyu Seon CHO
1
;
Hyeon Hoe KIM
;
Si Whang KIM
Author Information
1. Department of Urology, College of Medicine, Seoul National University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
MDCK(Madin-Darby canine kidney);
Oxalate;
Renal tubular epithelial cell
- MeSH:
Calcium Oxalate;
Cell Line;
Culture Media;
Epithelial Cells*;
Madin Darby Canine Kidney Cells;
Oxalic Acid;
Urinary Tract
- From:Korean Journal of Urology
1998;39(9):827-831
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We tried to evaluate the effect of oxalate on the growth of distal renal tubular epithelial cells MATERIALS AND METHODS: Madin-Darby canine kidney(MDCK) cell line, derived from canine distal renal tubular epithelial cell, supplied from American Type Culture Collection was used in this study. RPMI 1640 containing 10mM HEPES-buffer, 100IU/ microliterpenicillin, 100 microgram/ microliterstreptomycin, 300 microgram/ microliterglutamine, and with or without 10% fetal bovine serum(FBS) was used as the culture media. And the cell survivals under various concentrations of ammonium oxalate were assessed by MTT assay. RESULTS: Exposure of MDCK cells to oxalate resulted in a concentration dependent suppression of the cell growth in both 10% FBS supplied media and FBS deprived media. CONCLUSIONS: From these observations we could conclude that oxalate is not a mere constituent of calcium oxalate stone, but oxalate might offer a good environment for calcium oxalate stone formation in the urinary tract via MDCK cellular damage.
