Cultivation,identification and sorting of bronchoalveolar stem cells derived from mouse lung
- VernacularTitle:小鼠支气管肺泡干细胞的培养鉴定和分选
- Author:
Shen QIAN
;
Jianghong AN
;
Xujun AO
;
Jianguo SUN
;
Zhengtang CHEN
- Publication Type:Journal Article
- Keywords:
stem cells;
clara cell antigen;
pulmonary surfactant protein C;
flow cytometry
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To cultivate,identify and sort bronchoalveolar stem cells(BASC)derived from normal adult mouse lung.Methods After enzymatic digestion of lung tissue with dispase and collagenase in combination,the Sca-1+ cells were isolated from the obtained pulmonary cells by magnetic cell sorting.These Sca-1+ cells were cultured in dishes coated with collagen and mouse fibroblast cell line Swiss-3T3 under a serum-free culture system for BASC,which were identified by the dual-color immunofluorescent staining clara cell specific antigen(CCA)and surfactant protein C(SP-C).Finally,these pure BASC were isolated by the flow cytometry.Results One lung of normal adult mouse could yield(1.6-1.8)?107 nucleated cells in this enzyme digestion procedure.The percentage of Sca-1+ cells we sorted from lung tissue was much higher than the unsorted [(87.3?5.9)% and(9.6?1.8)%,P
