Determination of the Baicalin in Pharynx Preparation by HPLC
- VernacularTitle:HPLC法测定利咽合剂中黄芩苷的含量
- Author:
Yuyan XIAO
;
Na LIN
- Publication Type:Journal Article
- Keywords:
pharynx preparation;
baicalin;
HPLC;
content detection
- From:
Journal of Zhejiang Chinese Medical University
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] It is aimed at discovering an effective way of HPLC to detect the content of baicalin in the pharynx preparation.[Method] The column of Hypersil-ODS C18:250mm ? 4.6mm 5?m;the mobile phase:1.5% orthophosphoric acid and 10% methane alcohol;the detection wavelength: 276nm;velocity of flow: 1.0 ml/min;temperature: 40℃;the content is measured in the way of external standard method of apex area.[Result] By this method,baicalin takes on an excellent linearly relation within the range of 25.74?g?ml-1 ~ 514.8?g?ml-1,average recoveries get up to 99.8% and RSD 1.43% respectively.[Conclusion] This way is convenient and rapid,accurate and reliable,able to be used as a method of quality control on such baicalin preparation.
