Celastrol down-regulates expressions of MIF and MMP-9 in the aorta of ApoE gene knockout mice
- VernacularTitle:南蛇藤素对ApoE基因敲除小鼠主动脉壁MIF及MMP-9表达的影响
- Author:
Jinping LI
;
Jun CHENG
;
Zhuo TIAN
;
Houyuan HU
- Publication Type:Journal Article
- Keywords:
celastrol;
ApoE gene knockout mice;
macrophage migration inhibitory factor;
matrix metalloproteinase-9
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of celastrol on the expressions of macrophage migration inhibitory factor(MIF)and matrix metalloproteinase-9(MMP-9)in the aorta of apoE gene knockout mice with earlier atherosclerosis.Methods Eight-week-old ApoE gene knockout male mice were divided randomly into control group and celastrol treatment group(n=6 in each group).The mice in celastrol group were given.celastrol(2 mg?kg-1?d-1)by intraperitoneal injection for 4 weeks;and the mice in control group were only given equivalent amount of dimethyl sulfoxide(DMSO).HE staining of root aorta were used to observe the histomorphological changes and measure the size of plaque in ApoE-/-mice.The expressions of MIF and MMP-9 were detected by immunological histochemical method.Results The area of lipid plaque in the mice treated with celastrol was significantly smaller than that of the control(P
