Relationship between the expression of stromal interaction molecule 1 and the over proliferation of vascular smooth muscle cells in rats with injured carotid artery by balloon
- VernacularTitle:大鼠颈动脉球囊损伤后血管平滑肌细胞过度增殖与基质交感分子1的关系研究
- Author:
Ruiwei GUO
;
Lan HUANG
;
Pan GAO
- Publication Type:Journal Article
- Keywords:
carotid artery injuries;
smooth muscle, myoblasts;
stromal interaction molecule 1;
cell proliferation
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression changes of stromal interaction molecule 1 (STIM1) in vascular smooth muscle cells in balloon injury of carotid artery in rat in order to explore the proliferation mechanism of vascular smooth muscle cells. Methods A rat model of vascular restenosis induced by injury to the carotid arteries due to balloon dilatation was reproduced in present study. A total of 18 SD rats were randomly divided into 3 groups (6 each): control group, 7-day group and 14-day group after balloon injury to the carotid artery. The expression of STIM1 mRNA in vascular smooth muscle cells was assayed with reverse transcription polymerase chain reaction (RT-PCR), and the STIM1 protein was assessed by immunohistochemistry. The histopathology of the arteries was examined after by HE staining. Results Intima area/media area ratio (IA/MA) was significantly increased in 7-day group after balloon injury than in control group (P
