Dynamic changes in ATP-binding cassette transporter A1 in peripheral blood monocytes of patients with type 2 diabetes mellitus
- VernacularTitle:2型糖尿病患者外周血单核细胞三磷酸腺苷结合盒转运体A1的变化及其意义
- Author:
Jiayi WU
;
Shanshan ZHOU
;
Jie SHEN
- Publication Type:Journal Article
- Keywords:
diabetes, type 2;
lipoproteins, HDL cholesterol;
lipoproteins, VLDL cholesterol;
ATP-binding cassette transporters
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the dynamic changes in blood lipids and ATP-binding cassette transporter A1 (ABCA1) in peripheral blood monocytes of the patients with type 2 diabetes, and explore the correlations between ABCA1 expression and blood lipids levels and glycometabolism. Methods Human monocytes were isolated from the patients with type 2 diabetes (n=40) and from the healthy individuals (n=40) serve as normal control. ABCA1 was labeled by immune reaction PE fluorescence, then the changes in ABCA1 expression before and after being incubated with the ox-LDL were investigated by flow cytometer. The blood lipid levels, fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA1c) levels in the patients of type 2 diabetes group were determined, and the correlations between ABCA1 and age, blood lipid, and glycometabolic levels were analyzed. Results Plasma levels of triglyceride, non-high density lipoprotein cholesterol (NHDL-C) [low density lipoprotein cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C)] in the patients of type 2 diabetes group were significantly higher than that in control group (P
