Effects of propofol on the cytokine release from human monocytes induced by lipopolysaccharide
- VernacularTitle:异丙酚对脂多糖诱导人单核细胞释放细胞因子的影响
- Author:
Xugui CHEN
;
Miaoning GU
;
Zhuoqiang WANG
- Publication Type:Journal Article
- Keywords:
propofol;
lipopolysaccharide;
monocytes;
cytokine
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of propofol on the cytokine release from human monocytic cell line (THP1) induced by lipopolysaccharide (LPS). Methods THP1 cells cultured in vitro, and they were divided into 3 groups: 0 ?g/ml LPS group, 1 ?g/ml LPS group and 1?g/ml LPS+50?mol/L propofol group, respectively, and incubated for 12 hours. Then the supernatant was collected from each culture for the determination of the levels of granulocyte/macrophage colony stimulating factor (GM-CSF), interferon-? (IFN-?), tumor necrosis factor-? (TNF-?) and interleukin (IL)-1?, IL-2, IL-4, IL-6, IL-8, IL-10, and IL-12 by using LiquiChip system. Propol was added to the culture fluid of THP1 cells in the final concentrations of 0, 12.5, 25, 50 and 100?mol/L (for the groups B, C, D, E and F, respectively), with 1?g/ml LPS. 12 hours later the supernatants were collected to detect the levels of IL-6, IL-8, TNF-?. Culture fluid without LPS and propofol (group A) was used as control. Results The levels of IL-1?, IL-6, IL-8 and TNF-? were elevated obviously in group L than that in control group (P0.05). In group L+P, levels of IL-6, IL-8 and TNF-? were significantly lower than that in group L (P
