Correlation of monocyte counts prior to the collection of recombinant human granulocyte colony-stimulating factor primed bone marrow grafts with the quantities of CD34~+ cells in the grafts.
- VernacularTitle:rhG-CSF动员的健康供者骨髓采集当天外周血中单核细胞数量和骨髓采集物中CD34~+细胞数量关系的研究
- Author:
Yingjun CHANG
;
Mingrui HUO
;
Xiangyu ZHAO
;
Xiaohua LUO
;
Xiaojun HUANG
- Publication Type:Journal Article
- Keywords:
recombinant human granulocyte colony-stimulating factor;
hematopoietic stem cell transplantion;
CD34~+ cells;
monocyte
- From:
Chinese Journal of Practical Internal Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the correlation of monocyte counts prior to the collection of recombinant human granulocyte colony-stimulating factor(rhG-CSF)primed bone marrow grafts(G-BM)with the quantities of CD34+ cells in the grafts.Methods From June 2004 to July 2007,fifty-three healthy donors were treated with rhG-CSF[5 ?g/(kg?d)]injected subcutaneously for five consecutive days.Bone marrow grafts and peripheral blood grafts were harvested on the 4th and 5th day,respectively.The quantities of CD34+ cells and blood routine prior to the collection of the G-BM were determined by flow cytometry and blood analyzer XL2100,respectively.Results The monocyte counts in peripheral blood(PB)of the 53 healthy donors were 896?424 per microliter.The counts of CD34+ cells per microliter and quantities of total CD34+ cells in the bone marrow grafts were 84?45 and(8.22?4.84)?107,respectively.Pearson correlation analysis indicated that the counts of monocyte in the PB correlated positively with the counts of CD34+ cells per microliter(r=0.573,P
