Correlation of plasminogen activator and plasminogen active inhibitor expression and capillary density in IgA nephropathy.
- VernacularTitle:纤溶酶原激活物及其抑制剂-1表达与IgA肾病毛细血管密度相关关系的研究
- Author:
Qiang QIU
;
Xuefeng SUN
;
Xiangmei CHEN
;
Quan HONG
- Publication Type:Journal Article
- Keywords:
IgA nephropathy;
microvascular disease;
plasminogen activator;
plasminogen activator inhibitor-1
- From:
Chinese Journal of Practical Internal Medicine
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze mRNA and protein expressions of plasminogen activator and plasminogen activator inhibitor-1 using microdissection and quantitative real-time PCR,and to analyse their associations with microvascular diseases in IgA nephropathy.Methods Twenty-four IgA nephropathy patients treated in Kidney Center and Kidney Laboratory of PLA from 2000 to 2004 were admitted into the study;20 renal glomeruli or 200 renal tubules and peritubular interstitial per patient were captured by Laser Microdissection System from IgA nephropathy renal biopsy slides(8 ?m),and mRNA was extracted.PA mRNA and PAI-1 mRNA levels in glomerulus and tubulointerstitial area were measured by Taqman quantitative real-time PCR.Capillaries densities in glomerulus and tubulointerstitial area were measured using immunohistological staining method and computer image analysis system.Results With the aggravation of lesions,the glomeruli and peritubular capillaries densities of IgA nephropathy decreased.The glomerular and peritubular capillaries densities were negatively associated with the level of serum creatinine(R2=0.6946 and R2=0.6271,P
