Construction of MafA recombinant retroviral vector and its stable expression in liver epithelial progenitor cells
- VernacularTitle:MafA逆转录病毒表达载体的构建及其在肝原始细胞中的稳定表达
- Author:
Caixia JIN
- Publication Type:Journal Article
- Keywords:
MafA;
retroviral vector;
liver progenitor cells
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a recombinant retroviral vector harboring MafA and to establish a liver epithelial progenitor cell(LEPC)line for stable expression of MafA.Methods:MafA was amplified by PCR and suncloned into pBMN-Z- IRES-Neo vector to obtain pBMH-MafA-Neo vector.After introducing the pBMN-MafA-Neo into Phoenix package cells,the cell culture supernatant was used to infect LEPCs.LEPCs stably expressing MafA gene were screened out.RT-PCR was used to detect the influence of MafA on the phenotype of LEPCs.Results:We successfully constructed pBMH-MafA-Neo vector and obtained LEPCs which stably expressed MafA.Expression of GK and GLUT2 genes in LEPCs-MafA cells was higher than that in the LEPCs.Conclusion:We have successfully obtained LEPCs which can stably express MafA,laying a basis for studying the differentiation of LEPCs into pancreas cells.
