Growth inhibition effects of jasmonates on human neuroblastoma cell line SH-SY5Y and its mechanisms
- VernacularTitle:茉莉素对人神经母细胞瘤细胞株SH-SY5Y的生长抑制作用及其机制研究
- Author:
Guosong JIANG
;
Qiangsong TONG
;
Fuqing ZENG
;
Bo HU
;
Liduan ZHENG
;
Jiabin CAI
;
Yuan LIU
;
Zhaohui GU
;
Zhiyu WANG
- Publication Type:Journal Article
- Keywords:
jasmonates;
neuroblastoma;
cell cycle arrest;
apoptosis;
mechanisms
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the growth inhibition effects of jasmonates on human neuroblastoma SH-SY5Y cell line,and to investigate its mechanisms.Methods After administration of 0.5~2.5 mmol?L-1 jasmonates for 6~24 hrs, the growth inhibition rates of SH-SY5Y cells were studied by MTT colorimetry.Cell cycle phases were assayed by propidium iodide staining flow cytometry. Cellular apoptosis was inspected by Hoechst 33258 fluorescent staining and Annexin V-FITC and propidium iodide staining flow cytometry.Gene expressions of PCNA, cyclin D1 and N-myc were determined by reverse transcription polymerase chain reaction.Results Jasmonates inhibited the growth of SH-SY5Y cells in a dose-and time-dependent manner,while the methyl jasmonate was the most efficient. After administration of 0.5 to 2.5 mmol?L-1 of methyl jasmonate for 24 hrs,the growth inhibition rates of cells reached 5.75%~88.7%(P
