Concentrations of secreted protein acidic and rich in cysteine in serum and urine and its expression in kidney tissues of patients with IgA nephropathy
- VernacularTitle:富含半胱氨酸的酸性分泌糖蛋白在IgA肾病患者血、尿中的含量及肾组织中的表达
- Author:
Bibo WU
- Publication Type:Journal Article
- Keywords:
secreted protein acidic and rich in cysteine;
IgA nephropathy;
blood;
urine;
kidney
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the concentrations of secreted protein acidic and rich in cysteine(SPARC)in the serum and urine of patients with IgA nephropathy and its expression in the kidney tissues.Methods:The concentrations of SPARC, tumor necrosis factor-?(TNF-?),interleukin 1?(IL-1?),and interleukin 6(IL-6)in the serum and urine were measured with enzyme-linked immunosorbent assay(ELISA).The contents of'SPARC protein in the culture medium of human mesangial cell (HMC)and human renal tubular epithelial cell(HKC),which had been treated with IL-6,were determined by ELISA.The ex- pression and distribution of SPARC in IgA nephropathy and normal kidney tissues were observed by immunohistochemistry as- say.Results:The concentrations of SPARC in serum and urine of IgA nephropathy patients were higher than those of the normal control subjects([2.43?1.22]?g/ml vs[0.69?0.21]?g/ml,[7.73?2.81]?g/ml vs[1.17?1.03]?g/ml,P
