Congenital anti-candida of human vaginal epithelial cells
- VernacularTitle:阴道上皮细胞先天性抗假丝酵母菌的作用研究
- Author:
Zhaohui LIU
;
Lei CHEN
- Publication Type:Journal Article
- Keywords:
Candidiasis, vulvovaginal;
Defensins;
Pulmonary surfactant-associated protein A
- From:
Journal of Peking University(Health Sciences)
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: T o further investigate pathogenesis about vulvovaginal candi diasis (VVC). Methods: Human normal vaginal epithelial cells in vitro were cultured by tissue cultur e and keratinocyto serum-free medium. Passaging epithelial cells were coculture d with candida albicans medium in separate wells for 0, 3, 6, 12, 24, and 48 h. C ontr ol epithelial cells were cultured alone in separate wells. For examination o f cytokines and chemokines, at each time point, the coculture supernatant and th e control culture were collected. ELISA was applied to detect the levels of HBD -1, HBD-2 and SP-A protein expression in the vaginal epithelial cells (HBD-1 :F= 62.784,P=0.001; HBD-2: F=5127.984, P=0.000; F=542.210, P=0 .000). Also, we colle cted the vaginal doughing water of VVC patients and healthy women, and detected the levels of SP-A, then analysed the data. Results: In the tra il of human vagin al epithelial cells, in contrast to the control group, the medium group induced the epithelial cells to secrete more HBD-1, HBD-2 and SP-A at h48, which was co nsidered to be significant (P
