Influences of stemona alkaloids on esterase isozymes activities and glycogen content in Oncomelania hupensis
- VernacularTitle:百部总碱对钉螺酯酶同工酶活性及糖原含量的影响
- Author:
Qian CHEN
;
Wanxian WANG
;
Wenshan KE
;
Xu LIU
;
Xiuli XIANG
- Publication Type:Journal Article
- Keywords:
Stemona alkaloids;
Oncomelania hupensis;
Esterase isozymes;
Glycogen
- From:
Chinese Journal of Schistosomiasis Control
1989;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influences of stemona alkaloids on esterase isozymes activities and glycogen content of Oncomelania hupensis in order to explore the molluscicidal mechanism of stemona alkaloids.Methods O.hupensis snails were immersed in the liquid of stemona alkaloids at the concentration of 6.5 mg/L(72 h LC50),the surviving ones were picked out and sampled after being immersed for 12,24,48,72,96 h,then PAGE and anthrone colorimetry methods were used to observe the changes of liver esterase isozymes activities and tissue glycogen content of O.hupensis during the immersion period.A group of snails immersed in de-chlorinous water served as control.Results Esterase isozymes activities firstly increased,and then decreased until almost lost completely during the 96 h immersion period.Meanwhile,glycogen content gradually decreased as the immersion time extended.After being immersed for 96 h,glycogen content decreased by 72.00% compared with the control group.Conclusion Stemona alkaloids could inhibit the viability of O.hupensis by causing decrease of esterase isozymes activities and glycogen content.
