Identification of suppressive oligodeoxynucleotide existed in DNA sequence encoding Schistosoma japonicum 22.6 kDa antigen
- VernacularTitle:日本血吸虫22.6 kDa抗原编码基因序列中抑制性片段的确定
- Author:
Wei HU
;
Mingjuan TAN
;
Yong WANG
;
Yuejin LIANG
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
22.6 kDa antigen;
Immunostimulatory CpG 1826;
Suppressive oligodeoxynucleotide
- From:
Chinese Journal of Schistosomiasis Control
1989;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the possible existing suppressive oligodeoxynucleotides(ODNs)in the DNA sequence which encodes Schistosoma japonicum 22.6 kDa(Sj22.6)antigen.Methods Several ODNs within the DNA sequence encoding Sj22.6 antigen were synthesized.Splenocytes separated from mice were stimulated with optimal immunostimulatory CpG 1826 in the absence or presence of different synthetical ODNs.The suppressive efficacy of each ODN was examined by 3H-TdR incorporation.Results ODN F311 suppressed the proliferation of splenocytes caused by CpG 1826 stimulation.The significant suppression was observed when ODN F311∶CpG 1826 at a ratio of 1∶1 and 3∶1,the suppression reached 11% and 58% respectively.The maximal inhibition was observed when ODN F311 was pre-administered with CpG ODN for 2 h.Conclusions Certain suppressive ODN exists in the DNA sequence encoding Sj22.6 antigen,and this effect shows dose-and time-dependent manner.
