Application of Diagnosis of Pulmonary Tuberculosis with Phage Splitting Assay
- VernacularTitle:噬菌体裂解法在肺结核快速诊断中的应用
- Author:
Ruiru SHI
;
Feixu GAO
;
Jiangdong WANG
;
Aihua LI
;
Xin LIU
;
Guolong ZHANG
- Publication Type:Journal Article
- Keywords:
Phage;
Mycobacterium tuberculosis;
Tuberculosis
- From:
Journal of Medical Research
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish phage splitting method for Mycobacterium tuberculosis and explore its application in the diagnosis of pulmonary tuberculosis.Methods Two hundred and fifteen sputum specimens were tested by L-J medium culture method,fluorescent microscopy method and phage splitting method concurrently.Results Comparing with L-J medium method,the sensitivity and specificity of phage splitting method were 71.0%,97.9%,respectively,however for routine microscopic method,the sensitivity and specificity were 55.7% and 91.2%.Conclusion With very high specificity and comparatively better sensitivity,phage splitting method can rapidly detect Mycobacterium tuberculosis and can be used in the rapid diagnosis of pulmonary tuberculosis.
