Determination of Gatifloxacin Mesylate for Injection by HPLC
- VernacularTitle:高效液相色谱法测定注射用甲磺酸加替沙星含量
- Author:
Jing TIAN
- Publication Type:Journal Article
- Keywords:
Gatifloxacin mesilate for injection;
Determination;
HPLC
- From:
Journal of Medical Research
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method for the determination of gatifloxacin mesylate for injection.Methods The chromatographic condition was as follows:Determination was performed with C18(150mm?4.6mm,5?m)as the chromatographic column.The mobile phase was consisted of phosphoric acid-acetonitrile(82:18)with a flow rate at 1.0 ml/min.The column temperature was 30℃ and the detection wavelength was 325nm.Results Good linear relation was achieved when the gatifloxacin concentration was at the range of 78.8～630.4?g/ml(r=0.9999).At the concentration of 80% 、100%、120%,the mean recovery were 99.45%(RSD 1.29%,n=3)、99.24%(RSD 0.47%,n=3)、99.81%(RSD 0.75%,n=3).,respectively.Conclusion The method is accurate with a good reproducibility and can be used as a quantitative analys for preparation.
