esp1 expression and its effect on ploidy in A549 and Hela cells
- VernacularTitle:esp1在A549和Hela细胞中的表达及对染色体倍性的影响
- Author:
Lianzhong FU
;
Xin LIU
;
Chengde ZHANG
- Publication Type:Journal Article
- Keywords:
estradiol-stimulated protein;
ploidy;
aneuploidy;
over-expression
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the relation of estradiol-stimulated protein (esp1) expression with chromosome ploidy in A549 and Hela cells. Methods Total RNA was extracted from A549 and Hela cells respectively for cDNA. pIRE-S2-EGFP-ESP1 plasmid was transfected into the 2 corresponding tumor cells. Real-time quantitative PCR was used to detect esp1 gene expression. The chromosome changes were analyzed by VIDEOTEST-KARYO 3.1 and chromosome ploidy was analyzed with flow cytometry. Results After the tumor cells were transfected with pIRE-S2-EGFP-ESP1 plasmid, the expression of esp1 gene was up-regulated (P
