Effect of brain microvascular endothelial cells on differentiation of co-cultured mesenchymal stem cells under hypoxic conditions
- VernacularTitle:缺氧条件下大鼠脑微血管内皮细胞对共培养的人间充质干细胞分化的影响
- Author:
Kaiming LIU
;
Luxiang CHI
;
Xianghui LU
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
hypoxia;
co-culture;
differentiation;
brain microvascular endothelial cells
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of brain microvascular endothelial cells (BMECs) on mesenchymal stem cells (MSCs) expressing fetal liver kinase-1(Flk-1) and von Willebrand factor (vWF) under normal and hypoxic conditions. Methods Human bone marrow MSCs and rat BMECs were isolated, cultured and identified. We used direct and indirect co-culture to induce the differentiation of MSCs. The expression of Flk-1 and vWF after induction was observed and analyzed by flow cytometry and immunofluorescence staining. Results Undifferentiated MSCs did not express Flk-1 or vWF. In indirect co-culture group, indirect co-culture under hypoxic conditions induced (7.58?0.58)% (n=6, P=0.034) MSCs to express Flk-1 but not vWF, while in normoxic indirect co-culture, MSCs expressed neither. In direct co-culture group, after 5-day direct co-culture with BMECs under normoxia or hypoxia, MSCs expressing Flk-1 accounted for (13.76?1.67)% (n=6, P
