Connexin 43 expression in cultured interstitial cells of Cajal from guinea pigs
- VernacularTitle:豚鼠膀胱组织ICCs细胞间隙连接蛋白Connexin43表达意义
- Author:
Junan YAN
;
Gensheng LU
;
Longkun LI
;
Bo SONG
- Publication Type:Journal Article
- Keywords:
interstitial cells of Cajal;
gap junction protein;
bladder
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of connexin 43 in the interstitial cells of Cajal (ICCs) from guinea pig bladder in vitro. Methods Bladder ICCs were primarily cultured from guinea pigs by collagenase digestion method. Primarily cultured bladder smooth muscle cells (SMCs) from guinea pig were taken as control. The expressions of c-kit and smooth muscle actin (SMA) were detected by immunofluorescent method. Immumofluorescent method and Western blotting were used to detect the expression of Connexin 43 in 2 groups of cells. Results The expression of c-kit was positive in ICCs but negative in the control group. On the contrary, the expression of SMA was negative in ICCs but positive in the control group. Connexin 43 expression was significantly higher in ICCs than that in the control group (P
