Activity and mRNA expression of cytochrome P450 and fentanyl metabolism in rat intestine in anhepatic phase
- VernacularTitle:大鼠无肝状态小肠CYP3A1酶活性增强和mRNA表达增高及芬太尼肝外代谢
- Author:
Yuping LI
;
Jianteng GU
;
Guocai TAO
;
Xuefeng XIANG
;
Bo YANG
- Publication Type:Journal Article
- Keywords:
fentanyl;
CYP3A1;
anhepatic phase;
plasma concentration;
RT-PCR;
fluorometry
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the variation of fentanyl concentration and the gene expression and activity of rat intestinal cytochrome P450 3A1 in anhepatic phase. Methods The experiment was comprised of 2 steps. Step 1: The rats were randomly divided into experimental group (group A2, underwent occlusion of the hepatic portal) and control group (group A1), with 10 rats in each group. Fentanyl blood concentration was analyzed by LC/MS/MS. Step 2: The rats were randomly divided into group B1 (control), group B2 and group B3 (the rats underwent devascularization of the hepatic portal for 30 or 60 min). The levels of CYP3A1 in rat small intestine were assessed with RT-PCR and the enzymic activity of CYP3A1 was detected by fluorometry. Results Fentanyl concentration in anhepatic phase dropped more slowly in group A2 than group A1 (P
