Application of pulsed field gel electrophoresis on the karyotype analysis of Candida albicans
- VernacularTitle:脉冲场凝胶电泳在白色假丝酵母菌基因核型分析中的应用
- Author:
Hong LEI
;
Mei DONG
;
Bin SUN
- Publication Type:Journal Article
- Keywords:
Candida albicans;
electrophoresis, gel, pulsed-field;
karyotyping
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the karyotype of Candida albicans DNA by pulsed field gel electrophoresis (PFGE), and evaluate the significance of the karyotype used as a method for molecular epidemiology controlling. Methods 14 isolates of C. albicans from various clinical samples were evaluated for molecular relatedness by PFGE. Isolates were identified by MircroScanWalkAway 96 RY-ID. Results The karyotype analysis revealed a total of 9 different genotypes among 14 isolates of C. albicans from 11 patients. Among the isolates, 4 were isolated from difference parts of a patient and had same karyotype, implying that they were from the same isolate and differed to different sub-clones. In another 10 isolates from different patients, 3 had same karyotype, and the karyotypes of the remainder 7 isolates were different. Conclusion PFGE may well reveal the autoploidy and polymorphism of the isolates of C. albicans with different origin, thus may be used as an effective method for molecular epidemiological study.
