Determination simultaneously of gentiopicroside and geniposide in Longdan Xiegan Pill by HPLC
- VernacularTitle:HPLC同时测定龙胆泻肝丸中龙胆苦苷和栀子苷的含量
- Author:
Ruixin LIU
- Publication Type:Journal Article
- Keywords:
HPLC;
Longdan Xiegan Pill;
gentiopicroside;
geniposide
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a HPLC method for determining simultaneously gentiopicroside and geniposide in Longdan Xiegan Pill(Radix et rhizoma Gentianae,Fructus Gardeniae,Radix Scutellariae,etc.).METHODS:C_ 18 column(5 ?m,4.6 mm?250 mm)was used.The mobile phase was methanol-water(23∶77).The detecting wavelength and the temperature of column were at 238 nm and at 40 ℃ respectively.RESULTS:The linear detection range of gentiopicroside was 3.03-30.30 ?g/mL(r=1.000 0),and the average recovery was 98.43%(RSD=1.08%).The linear detection range of geniposide was 9.76-97.60 ?g/mL(r=0.999 9),and the average recovery was 99.95%(RSD=0.91%).CONCLUSION:Two ingredients in Longdan Xiegan Pill could be determined simultaneously by this method which is convenient,rapid and accurate.It can be used for quality control in the production of Longdan Xiegan Pill.
