Relationship between antitumor activity of Gefitinib and epidermal growth factor receptor expression in human colorectal cancer cell lines
- VernacularTitle:Gefitinib对人结直肠癌细胞的生长抑制作用与表皮生长因子受体表达关系的研究
- Author:
Li YANG
;
Yuying CHEN
;
Feng PAN
- Publication Type:Journal Article
- Keywords:
Gefitinib;
colorectal neoplasms;
cell line, tumor;
epidermal growth factor, receptor;
drug screening assay, antitumor
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antitumor activity of Gefitinib, a selected epidermal growth factor receptor-tyrosine kinase inhibitor, on human colorectal cancer cell lines in vitro, and to explore the relationship between the inhibitory effect of Gefitinib on cancer cells and the expression of epidermal growth factor receptor (EGFR). Methods The growth inhibitory effects of Gefitinib, which expressed as the half growth inhibition dose IC50, on colorectal cancer cells were assessed by MTT assay. EGFR mRNA expression was detected by reverse transcriptional PCR (RT-PCR). Western blot was used to determine the expression of EGFR protein as well as its phosphorylated forms (p-EGFR). Results Gefitinib inhibited growth of all the six colorectal cancer cell lines in vitro with an IC50 range from 6.5 to 172.7?mol/L. Lovo cell line, with an IC50 value less than 10?mol/L, was the most sensitive one to Gefitinib, HT29 and SW480 were moderate sensitive to 10?mol/L
