Effect of powder of Chinese Angelica and Peony on expressions of hippocampal proteins in senescence accelerated mice
- VernacularTitle:当归芍药散对快速老化模型小鼠海马蛋白表达的影响
- Author:
Miao GENG
;
Ning JIANG
;
Wenxia ZHOU
;
Yongxiang ZHANG
- Publication Type:Journal Article
- Keywords:
powder of Chinese Angelica and Peony;
senescence accelerated mouse;
hippocampus;
proteomics
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of powder of Chinese Angelica and Peony (DSS) on hippocampus proteome of senescence accelerated mice (SAM). Methods Thirty female mice aged 12 months were used in our study, including 10 SAM-resistance/1 (SAMR1) and 20 SAM-prone/8 (SAMP8). The difference of hippocampus proteome among SAMP8, SAMR1, SAMP8 treated with DSS intragastrically at dose of 3.2 g/kg once a day for 30 d (SAMP8+DSS), was analyzed by two dimensional polyacryalmide gel electrophoresis (2DE). Results Compared with SAMR1, the expressions of 13 proteins in hippocampus of SAMP8 were up-regulated and that of 9 proteins were down-regulated significantly. Compared with SAMP8, the expressions of 21 proteins in hippocampus of SAMP8+DSS were up-regulated and that of 14 proteins were down-regulated significantly. Using MALDI-TOF-MS, the proteins with significant changes in expressions were identified by peptide fingerprinting map and the results were searched in MASCOT database, and sorted into 5 function groups including mitochondria-associated proteins, energy metabolism proteins, synaptic plasticity formation proteins, cytoskeletal proteins and others proteins. Conclusion The effects of DSS on hippocampus proteome of senescence accelerated mice were probably exerted via multi-target and multi-pathway mechanism.
