Morphological observation of embryonic stem cell-derived neurons after transplanted into A?-injured rat hippocampus
- VernacularTitle:神经前体细胞移植A?损伤大鼠海马后分化神经元的形态学观察
- Author:
Zhifang LI
;
Jun TANG
;
Lusi LI
;
Li YANG
;
Haiwei XU
- Publication Type:Journal Article
- Keywords:
embryonic stem cell;
neural precursor cell;
cell transplantation;
differentiation
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the morphology of embryonic stem cell-derived neurons after transplanted into A?-injured rat hippocampus. Methods Neural precursor cells (NPCs) were generated from mouse embryonic stem cells (ESCs) expressing EGFP with modified serum-free methods and then transplanted into the hippocampus of A?1-40-injuried rats. The morphology, neurotransmitter phenotypes and receptors of EGFP-positive donor cells were observed with immunofluorescene methods. Results The engrafted NPCs survived and differentiated into glutamatergic and GABAergic neurons and expressed both glutamatergic and GABAergic neurotransmitter receptors. Synaptophysin-positive dots were found surrounding somata and dendrites of transplanted neurons, suggesting the presence of presynaptic terminals adjacent to their membranes. Conclusion NPCs derived from ESCs can differentiate into excitatory and inhibitory neurons after grafted into Alzheimer's disease model rats, and maybe form synapse with the host neurons.
