Effects of hPDGF-A/hBD_2 genes transfection on rat bone marrow mesenchymal stem cells
- VernacularTitle:hPDGF-A/hBD_2双基因转染对大鼠骨髓间充质干细胞生物学特性的影响
- Author:
Yimei FENG
;
Hui XU
;
Zhongmin ZOU
;
Lei HAO
;
Tao WANG
;
Jun DENG
;
Shiwu DONG
;
Yongping SU
;
Tianmin CHENG
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cells;
gene transfection;
cell biology
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biological characteristics of platelet-derived growth factor A and human beta defensin 2 (hPDGF-A/hBD2) gene-modified rat bone marrow mesenchymal stem cells (BMSCs). Methods By using liposome transfection technique, recombinant adenovirus vector expressing hPDGF-A/hBD2 (Adv-hPDGF-A-IRES-hBD2) labeled with GFP was transfected into 293T cells for virus packaging and amplification. BMSCs were isolated, cultured and infected by adenovirus-containing supernatant. The exogenous gene-modified BMSCs were comprehensively studied on their biological features, in terms of morphology, cell growth curve, cell cycle, and adipogenic, osteogenic and myogenic differentiation ability. Results hPDGF-A-IRES-hBD2 gene-modified BMSCs did not show obvious changes in cell viability, proliferation, cell cycle distribution or cell differentiation. Conclusion BMSCs were not only good carriers for exogenous hPDGF-A and hBD2 genes but also seed cells for cell therapy even after hPDGF-A/hBD2 modification.
