RNAi silencing MTA1 gene inhibits invasion and migration of esophageal carcinoma 9706 cells
- VernacularTitle:RNAi干扰食管癌EC9706细胞MTA1基因表达对侵袭和迁移的影响
- Author:
Songhua YANG
;
Guoqiang ZHAO
;
Hong ZHENG
;
Jimin ZHAO
;
Ziming DONG
- Publication Type:Journal Article
- Keywords:
MTA1;
RNA interference;
invasion;
migration
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of RNAi that silences MTA1 gene on invasion and migration of esophageal carcinoma 9706 cells. Methods The siRNA expression vector that silences MTA1 gene was transfected into EC9706 cells by liposome. MTA1 mRNA and protein expressions were detected through quantitative RT-PCR and Western blot, respectively. The invasion and migration of EC9706 cells were evaluated by scrape wound healing assay and cell invasion assay in vitro. Results MTA1 gene expression significantly decreased. The scrape wound of EC9706 cells healed more slowly and the cell population that cut through Matrigel were less in the EC9706 cells transfected with siRNA expression vector than non-transfected EC9706 cells and the EC9706 cells transfected with blank vector (P
