Effect of CCK-8 on IL-12 secreted in murine bone marrow-derived dendritic cell induced by LPS
- VernacularTitle:CCK-8对LPS诱导小鼠骨髓来源树突状细胞分泌IL-12的影响
- Author:
Dongyan HAN
;
Bin CONG
;
Shujin LI
;
Xiaoli LIU
;
Chunling MA
;
Zhiyu NI
;
Yuxia YAO
;
Fenghua ZHANG
- Publication Type:Journal Article
- Keywords:
cholecystokinin;
dendritic cell;
interleukin-12
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of cholecystokinin-octopeptide on IL-12 secreted in murine bone marrow-derived dendritic cells induced by lipopolysaccharide.Methods The CCK receptor subtypes were investigated by immunofluorescence in murine bone marrow-derived dendritic cells.Enzyme linked immunosorbent assay and Western blot were used to estimate the contents of IL-12 and p38MAPK activity.Results CCK-1R and CCK-2R were detected in BM-DC;CCK-8(at concentrations of 10-10,10-8,10-6 mol?L-1)could significantly increase the secretion of IL-12 in the LPS-induced DC, and LPS-activated p38MAPK activity in a dose-dependent manner.The effect of CCK-8 was reduced partially by CR1409(a CCK-1R antagonist) and CR2945(a CCK-2R antagonist).Conclusion CCK-8 could dose-dependently increase the expressions of IL-12 in LPS-induced DC,probably by promoting p38MAPK activity and the effect was mediated by CCK1 and CCK2 receptors.
