Research of enalaprilat on neonatal rat cardiac fibroblasts proliferation and its mechanism
- VernacularTitle:依那普利拉对新生鼠心室成纤维细胞增殖的影响
- Author:
Hongxia SUN
;
Shijie YANG
- Publication Type:Journal Article
- Keywords:
enalaprilat;
angiotensin Ⅱ;
cardiac fibroblast;
cell cycle inhibitory protein p27kip1
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of angiotensin-converting enzyme inhibitor Ena on proliferation and cell cycle protein of cardiac fibroblasts and to explore the mechanism of Ena on cardiac fibrosis.Methods CFb was isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,collagen synthesis was observed by hydroxyproline concentration method,flow cytometry,immunofluorescenic and Western blot was used to measure cell cycle and CKI p27kip1 with Ena.Results Ena decreased MTT value and collagen synthesis dramatically;Ena increased phase G0/G1 and decreased phase S percentage ratio in cell cycle;Ena enhanced p27kip1 protein expression in a dose-dependent manner.Conclusion The antiproliferative effects of Ena on CFb can be attributed to upregulating CKI p27kip1 protein expression.
