Effect of ERK pathway inhibitor U0126 on cell cycle of leukemia K562 cell lines
- VernacularTitle:ERK阻滞剂U0126对白血病细胞K562细胞周期的作用及机制
- Author:
Xiao GUO
;
Ling PAN
- Publication Type:Journal Article
- Keywords:
leukemia;
cell cycle;
extracellular signal-regulated kinase;
K562 cells;
U0126
- From:Journal of Third Military Medical University
1988;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mechanism of extracellular signal-regulated kinase (ERK) pathway inhibitor U0126 on cell cycle of K562 cell lines. Methods The mRNA and protein expressions of ERK, Cyclin D2, Cyclin E in and P27 K562 cell lines after 10 ?mol/L U0126 treatment for 12, 24 and 72 h were detected by RT-PCR and Western blot, respectively. The cell cycle was determined by flow cytometry. Results The mRNA and protein expressions of ERK, Cyclin D2 and Cyclin E in K562 cell lines after U0126 treatment were decreased, while those of P27 were increased. The percentage of cells in G0/G1 phase was increased and that in S phase was decreased. All data mentioned above showed significant difference between the K562 cell lines before and after U0126 treatment(P
